Boettcher, Alfred and Moellers, Christoph and Lackner, Karl J. and Schmitz, Gerd (1998) Automated free-solution isotachophoresis: Instrumentation and fractionation of human serum proteins. ELECTROPHORESIS, 19 (7). pp. 1110-1116. ISSN 0173-0835,
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An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 mu m inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FREE-FLOW ISOTACHOPHORESIS; ELECTROPHORESIS; PURIFICATION; ACID; preparative isotachophoresis; human serum proteins |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 May 2023 09:07 |
| Last Modified: | 09 May 2023 09:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49751 |
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