Jendraschak, Ellen and Kaminski, Wolfgang E. (1998) Isolation of human promoter regions by Alu repeat consensus-based polymerase chain reaction. GENOMICS, 50 (1). pp. 53-60. ISSN 0888-7543,
Full text not available from this repository.Abstract
Knowledge of the promoter structure is critical for an understanding of the regulation of genes. We demonstrate by analysis of 405 human genes that human promoter regions are flanked by upstream Alu repeat elements, typically at a distance of 0.5-5 kb from their protein-coding areas. We identified common Alu repeat consensus sequences (ARC) among the different members of the Alu subfamilies that can be used as universal anchor sites for polymerase chain reaction (PCR) amplification. Utilizing ARC-specific primers and oligonucleotides specific for the 5' end of a selected target gene, we show that sequences spanning unknown human gene promoter regions can be directly amplified by PCR from genomic DNA. This novel technique, termed ARC-PCR, allowed us to characterize the proximal promoters of the human LTA(4) hydrolase and SPARC genes, each within 1 day. (C) 1998 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN MONONUCLEAR-CELLS; FACTOR MESSENGER-RNA; A(4) HYDROLASE GENE; HUMAN GENOME; REPEATED SEQUENCES; MOLECULAR-CLONING; RAPID ISOLATION; PCR; DNA; FAMILY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 May 2023 09:50 |
| Last Modified: | 23 May 2023 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49808 |
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