Loescher, F. and Ruckstuhl, T. and Jaworek, T. and Wegner, G. and Seeger, Stefan (1998) Immobilization of biomolecules on Langmuir-Blodgett films of regenerative cellulose derivatives. LANGMUIR, 14 (10). pp. 2786-2789. ISSN 0743-7463,
Full text not available from this repository. (Request a copy)Abstract
We present a new and fast method for preparation of ultrathin layers of biomolecules on solid substrates and its application as a useful tool for sensing biomolecules in an affinity biosensor. Nonamphiphilic trimethylsilyl ether-cellulose monolayers are used as interfaces to reduce nonspecific interactions between solid surfaces and biomolecules. These films are transferred on optical waveguides by the Langmuir-Blodgett technique. After their stabilization by cleavage of the hydrophobic trimehylsilyl groups, the cellulose films serve as excellent matrixes for the immobilization of proteins at high density. Cyanurchloride serves as a cross-linker between the cellulose layer and the proteins. The activity and biological specificity of these interfaces is controlled by an enzymatic assay and direct sensing by an evanescent wave immunosensor.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ULTRATHIN FILMS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 May 2023 09:54 |
| Last Modified: | 23 May 2023 09:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49811 |
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