Polus, Anna and Kiec-Wilk, Beata and Razny, Urszula and Gielicz, Anna and Schmitz, Gerd and Dembinska-Kiec, Aldona (2015) Influence of dietary fatty acids on differentiation of human stromal vascular fraction preadipocytes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1851 (9). pp. 1146-1155. ISSN 1388-1981, 0006-3002
Full text not available from this repository. (Request a copy)Abstract
Mediators such as cytokines, eicosanoids, nitric oxide and growth factors may regulate adipogenesis as well as inflammation. It is well documented that production of some form of eicosanoids activates lipid synthesis during adipogenesis but also contributes to the formation of factors maintaining low-level systemic inflammation. Developing nutrients for reduction of adipogenesis and inflammation can enhance preventive efficacy of daily diet. This study examined the effects of free fatty acid influence on changes in lipid biosynthesis and corresponding gene expression during differentiation of human subcutaneous adipose tissue stromal vascular fraction (SW) cells. Proadipogenic conditions promoted SW cell differentiation and lipid droplet (LD) formation up to 15 days. This correlated with gene expression of adipocyte differentiation markers as well as inflammatory cytokines and their receptors. Addition of free fatty acids to differentiation medium increased their incorporation during the first period of differentiation (48 h). Presence of eicosanoid acid (EPA) during the initial period of differentiation by elevation of Perilipin 3 protein (TIP47), may be responsible for smaller LD formation. Presence of arachidonic acid (AA) tends to deposit lipids in large form of LDs. Prolongation of differentiation up to 15 days decreased AA or EPA in cellular lipids. PUFA through up-regulation of both phospholipase 2 and enzymes related to eicosanoid production influenced type and quantity of eicosanoids which regulated the extent of SVF cell differentiation. Formation of small LDs and reduction of pro-inflammatory mediators in adipose tissue are the consequence of eicosanoid production with anti-inflammatory potential from EPA. (C) 2015 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOPLASMIC-RETICULUM STRESS; LIPID DROPLET FORMATION; ADIPOSE-SPECIFIC PHOSPHOLIPASE; TANDEM MASS-SPECTROMETRY; MESENCHYMAL STEM-CELLS; EICOSAPENTAENOIC ACID; 15-HYDROPEROXYEICOSAPENTAENOIC ACID; 3T3-L1 ADIPOCYTES; ARACHIDONIC-ACID; ADIPOGENIC DIFFERENTIATION; SVF; Adipogenesis; Lipidomics; Microarray; EPA; Eicosanoids |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Jun 2019 08:00 |
| Last Modified: | 14 Jun 2019 08:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4982 |
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