Kloth, Sabine and Gmeiner, Tanja and Aigner, Joachim and Jennings, Michael L. and Roeckl, W. and Minuth, Will W. (1998) Transitional stages in the development of the rabbit renal collecting duct. DIFFERENTIATION, 63 (1). pp. 21-32. ISSN 0301-4681, 1432-0436
Full text not available from this repository. (Request a copy)Abstract
The collecting duct (CD) epithelium of the mammalian kidney is an extraordinary structure with respect to its functional changes during development and its heterogeneous composition when matured. All of the different nephron epithelia of the mammalian kidney consist of one single cell type. In contrast, the differentiated CD is composed of at least three distinct cell types [principal, alpha intercalated-, and beta intercalated cells] that are responsible for the multiple physiological functions of this kidney compartment. During development the function of the CD changes: initially, the CD ampulla serves as an embryonic inducer, while the matured epithelium plays a key role in maintaining the homeostasis of body fluids. At present the process of CD maturation is not well understood. Neither the time course of development nor the morphogenic factors leading to the heterogeneously composed epithelium are known. In the present study the differentiation of the CD epithelium was investigated using newly developed monoclonal antibodies and well-characterized antisera. The morphological changes induced during differentiation were monitored by immunohistochemistry and scanning electron microscopy. The experiments were performed on neonatal and adult rabbit kidneys. Results obtained by light microscopical techniques and scanning electron microscopy revealed that the ampullary tip can be distinguished from the ampullary neck, as well as from the maturing CD, A number of proteins that were not detectable in the ampulla were detected in the neonatal CD and were found at even higher concentrations in the adult CD (P(CD)8, chloride/bicarbonate exchanger). Other proteins (P(CD)9) were downregulated during differentiation. For the first time the transient character of the differentiation stage of the neonatal CD could be demonstrated unequivocally. Furthermore, considerable heterogeneity in protein expression patterns (P(CD)6 and P(CD)9) was demonstrated within the beta IC cell population of the mature CD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERCALATED CELLS; POSTNATAL MATURATION; RAT-KIDNEY; IMMUNOCYTOCHEMICAL LOCALIZATION; MONOCLONAL-ANTIBODIES; BAND-3 PROTEIN; DIFFERENTIATION; PRINCIPAL; EXPRESSION; TRANSPORT; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 May 2023 10:44 |
| Last Modified: | 23 May 2023 10:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49831 |
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