Brunner, Anette and Minamitake, Yoshiharn and Goepferich, Achim (1998) Labelling peptides with fluorescent probes for incorporation into degradable polymers. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 45 (3). pp. 265-273. ISSN 0939-6411,
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Two peptides, atrial natriuretic peptide (ANP) and salmon calcitonin (sCT) were conjugated with a fluorescent, amine-reactive probe 5-(and 6-)carboxytetramethylrhodamine,-succinimidylester (5-(6)-TAMRA-SE). The labelling reaction was followed by HPLC and found to be complete after 2 h. The labelled peptides were purified by gel filtration chromatography and characterised by [H-1]NMR, UV/VIS and fluorescence spectroscopy. NMR-spectra confirmed the conjugation of dye to the peptides. Two absorption maxima between 500 and 600 nm were recorded in the UV/VIS-spectra. The fluorescence spectra were found to be pH-dependent, which allowed the measurement of pH in aqueous solution. The labelled peptides were encapsulated into poly(lactic acid) (PLA) microspheres using a double emulsion technique. Probe attachment permitted location of the peptides in the polymer. (C) 1998 Elsevier Science B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONTROLLED DELIVERY SYSTEMS; MICROSPHERES; PROTEINS; DERIVATIVES; peptide labelling; fluorescent bioconjugate; fluorescence; pH; degradable polymer; microsphere; poly(lactic acid) |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 May 2023 12:55 |
| Last Modified: | 23 May 2023 12:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49833 |
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