Ulbrecht, Matthias and Modrow, Susanne and Srivastava, Rakesh and Peterson, Per A. and Weiss, Elisabeth H. (1998) Interaction of HLA-E with peptides and the peptide transporter in vitro: Implications for its function in antigen presentation. JOURNAL OF IMMUNOLOGY, 160 (9). pp. 4375-4385. ISSN 0022-1767,
Full text not available from this repository. (Request a copy)Abstract
The assembly of MHC Ia molecules in the endoplasmic reticulum requires the presence of peptide ligands and beta(2)m and is facilitated by chaperones in an ordered sequence of molecular interactions. A crucial step in this process is the interaction of the class I alpha-chain/beta(2)m dimer wvith TAP, which is believed to ensure effective peptide loading of the empty class I molecule, We have previously demonstrated impaired intracellular transport of the class Ib molecule HLA-E in mouse myeloma cells cotransfected with the genes for HLA-E and human beta(2)m, which is most likely attributable to inefficient intracellular peptide loading of the HLA-E molecule. We therefore analyzed the ability of HLA-E in the transfectant cell line to bind synthetic peptides by means of their ability to enhance cell surface expression of HLA-E, Peptide binding was confirmed by testing the effect on the thermostability of soluble empty HLA-E/human beta(2)m dimers, Two viral peptides binding to HLA-E were thus identified, for which the exact positioning of the N terminus appeared critical for binding, whereas the contribution of the length of the C terminus seemed to be minor, allowing peptides as short as seven amino acids and up to 16 amino acids to exhibit considerable binding activity. Furthermore, we demonstrate that HLA-E interacts with TAP and that this interaction can be prolonged by the proteasome inhibitor N-acetyl-L-leucyl-L-leucyl-L-norleucinal which reduces the intracellular peptide pool. The presented data indicate that HLA-E is capable of presenting peptide ligands similar to the repertoire of HLA class Ia molecules.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CLASS-I MOLECULES; HISTOCOMPATIBILITY COMPLEX-MOLECULES; DROSOPHILA-MELANOGASTER CELLS; T-CELLS; ENDOGENOUS PEPTIDES; NONCLASSICAL MHC; BINDING; EXPRESSION; TAP; ANCHOR; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 May 2023 13:16 |
| Last Modified: | 23 May 2023 13:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49856 |
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