Eisinger, Kristina and Froehner, Stanley C. and Adams, Marvin E. and Krautbauer, Sabrina and Buechler, Christa (2015) Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin. ANALYTICAL BIOCHEMISTRY, 484. pp. 99-101. ISSN 0003-2697, 1096-0309
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Alpha-syntrophin (SNTA) is an adaptor protein that regulates several signaling pathways. To analyze expression of SNTA immunoblot assays must be performed. Here, the specificity of four commercially available SNTA antibodies has been evaluated in immunoblot experiments using liver tissues of wild-type and SNTA-deficient mice. While one of the antibodies reacts with SNTA, two antibodies specifically recognize beta 2 syntrophin (SNTB2). The antigen detected by the fourth antibody has not been identified but is different from SNTA and SNTB2. Therefore, only one of the four tested antibodies is appropriate to analyze SNTA protein levels by immunoblot. (C) 2015 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEINS; LOCALIZATION; MUSCLE; ABCA1; Immunoblot; Knockout mice; Liver |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Jun 2019 08:10 |
| Last Modified: | 14 Jun 2019 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4986 |
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