Pietzsch, Anett and Buechler, Christa and Schmitz, Gerd (1998) Genomic organization, promoter cloning, and chromosomal localization of the Dif-2 gene. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 245 (3). pp. 651-657. ISSN 0006-291X,
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We describe the genomic organization and the functional promoter of the monocyte specific gene Dif-2, the human homologue to genes in mouse (gly96) and rat (PRG1), that is downregulated during cell differentiation. The Dif-2 gene consists of two exons and a single intron of 112 bp in length. RNase protection assay indicates one major transcription start site. Sequence analysis reveals several consensus sequences for transcription factors including NF-kappa B, C/EBP, SPI, and the lack of a classical TATA-box. To demonstrate promoter activity, DNA fragments of the Dif-2 5'-flanking region were ligated upstream to the luciferase gene and transfected into HepG2 and HeLa cells. A minimal promoter element between nt -158 and nt +74 containing NF-kappa B and SPI binding sites was shown to be sufficient for basal activity. These transcription factor binding sites, which are conserved between Dif-2, gly96, and PRG1 promoter regions, indicate a significant role for Dif-2 expression and may explain LPS and Cz-ceramide sensitivity. The Dif-2 gene was mapped to chromosome 6p21.3 using in situ hybridization technique. (C) 1998 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EARLY-RESPONSE GENE; INSITU HYBRIDIZATION; BINDING PROTEIN; MACROPHAGES; CELLS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jun 2023 12:27 |
| Last Modified: | 20 Jun 2023 12:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49888 |
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