Dragon, Stefanie and Hille, Rainer and Goetz, Robert and Baumann, Rosemarie (1998) Adenosine 3 ': 5 '-cyclic monophosphate (cAMP)-inducible pyrimidine 5 '-nucleotidase and pyrimidine nucleotide metabolism of chick embryonic erythrocytes. BLOOD, 91 (8). pp. 3052-3058. ISSN 0006-4971,
Full text not available from this repository. (Request a copy)Abstract
Terminally differentiating erythrocytes degrade most of their RNA with subsequent release of mononucleotides. Pyrimidine mononucleotides are preferentially cleaved by an erythrocyte-specific pyrimidine 5'-nucleotidase; deficiency of this enzyme causes hemolytic anemia in humans, Details of the regulation of its activity during erythroid differentiation are unknown. The present study arose from the observation that the immature red blood cells (RBCs) of mid-term chick embryos contain high concentrations of uridine 5'-triphosphate (UTP) (5 to 6 mmol/L), which decline rapidly from days 13 to 14 onward. We analyzed two key enzymes of RBC pyrimidine nucleotide metabolism: pyrimidine nucleoside phosphorylase (PNP) and pyrimidine 5'-nucleotidase (P-5'-N), to evaluate if changes of enzyme activity during embryonic development are correlated with changes of RBC UTP. Secondly, we tested if these enzymes are under hormonal control. The results show that embryonic RBCs contain only minimal activity of PNP. In contrast, P-5'-N increases from day 13 on, suggesting that the enzyme is a limiting factor in UTP degradation. Activation of beta-adrenergic and A(2A)-adenosine receptors causes transcription dependent de novo synthesis of P-5'-N, Because beta-adrenergic and adenosine receptors are also found on adult erythroid cells, P-5'-N might be an enzyme of differentiating RBCs whose expression is in part controlled by adenosine 3':5'-cyclic monophosphate (cAMP). (C) 1998 by The American Society of Hematology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKELETAL-MUSCLE CELLS; RED-BLOOD-CELLS; PRIMARY CULTURE; BIOSYNTHESIS; CARNOSINE; RECEPTOR; OXYGEN; ASSAY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Alumni or Retired > Prof. Dr. Rosemarie Baumann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jun 2023 12:44 |
| Last Modified: | 20 Jun 2023 12:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49897 |
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