Froehlich, Dieter and Spertini, O. and Moser, R. (1998) The Fc gamma receptor-mediated respiratory burst of rolling neutrophils to cytokine-activated, immune complex-bearing endothelial cells depends on L-selectin but not on E-selectin. BLOOD, 91 (7). pp. 2558-2564. ISSN 0006-4971,
Full text not available from this repository.Abstract
Intracellular H2O2 generation, as a measure of the respiratory burst, was determined after stimulation of neutrophils by immune complex (IC)-bearing human umbilical vein endothelial cells. Under static conditions, neutrophils basically responded to the immune deposits on resting endothelial cells. The rotating shear forces of approximate to 0.7 dynes/cm(2), corresponding to the physiological flow in postcapillary venules, completely abolished this basal H2O2 generation, After activation of the IC-bearing endothelial layers with interleukin-1 (IL-1) or tumor necrosis factor (TNF), or both, for 4 hours, rolling adhesion of the neutrophils was induced, accompanied by considerable H2O2 production. The neutrophil respiratory burst was prominently inhibited by anti-Fc gamma RIII MoAb 3G8 (72.4%), and partially by MoAb 2E1 against Fc gamma RII (38.5%). Both MoAbs together inhibited the Fc-mediated H2O2 generation by 93.4%. The respiratory burst and rolling adhesion were markedly blocked by MoAb LAM1-3 against L-selectin (91.3%), whereas the nonfunctional anti-L-selectin MoAb LAM1-14 was ineffective. F(ab)(2)' fragments of MoAb 7A9 against E-selectin inhibited neutrophil rolling by 98.6%, but not the respiratory burst. Moreover, rolling adhesion of neutrophils and the related oxidative burst were CD11b/CD18- independent. In summary, L-selectin has a unique auxiliary function in triggering the Fc gamma R-mediated respiratory tory burst of rolling neutrophils to IC-bearing endothelial cells, thereby substituting CD11b/CD18 under conditions of flow. (C) 1998 by The American Society of Hematology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LEUKOCYTE ADHESION MOLECULE-1; P-SELECTIN; GLYCOPROTEIN LIGAND-1; SURFACE EXPRESSION; TYROSINE PHOSPHORYLATION; HYDRODYNAMIC SHEAR; OXIDATIVE BURST; III CD16; IN-VITRO; MAC-1; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Sep 2023 14:49 |
| Last Modified: | 05 Sep 2023 14:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49952 |
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