Otterbein, C. K. and Splettstoesser, W. D. and Linde, H. J. and Grunow, R. and Wolf, H. and Finke, E. J. and Neubauer, H. (1998) Development and characterization of a murine monoclonal antibody reactive with a 64 kDa somatic antigen of Burkholderia cepacia. HYBRIDOMA, 17 (2). pp. 143-150. ISSN 1554-0014, 1557-8348
Full text not available from this repository.Abstract
Monoclonal antibodies (MAbs) to Burkholderia cepacia were produced from mice immunized with inactivated whole-cell antigen. For screening of resulting MAbs an enzyme-linked immunosorbent assay (ELISA) was used. A stable hybridoma cell line (BC-2) producing specific antibodies to a 64 kDa somatic antigen from B, cepacia was established, In ELISA and immunoblotting analysis the MAb BC-2 recognized all tested strains of B, cepacia whereas no cross-reaction with 32 Pseudomonas aeruginosa strains was found. From a wide range of other bacteria only strains of the species Burkholderia mallei, Burkholderia pseudomallei, and Burkholderia gladioli showed cross-reactions. The MAb BC-2 will be used to develop a diagnostic assay for the identification of B, cepacia and B. gladioli, important agents of nosocomial infections in immunocompromised patients suffering especially from cystic fibrosis (CF).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYSTIC-FIBROSIS PATIENTS; PSEUDOMONAS-CEPACIA; SP-NOV; COMB-NOV; GEN-NOV; PSEUDOMALLEI; IDENTIFICATION; TRANSMISSION; MELIOIDOSIS; RESISTANCE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2023 06:29 |
| Last Modified: | 12 Sep 2023 06:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49964 |
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