Ammon, C. and Kreutz, Marina and Rehli, M. and Krause, S. W. and Andreesen, R. (1998) Platelets induce monocyte differentiation in serum-free coculture. JOURNAL OF LEUKOCYTE BIOLOGY, 63 (4). pp. 469-476. ISSN 0741-5400, 1938-3673
Full text not available from this repository. (Request a copy)Abstract
Terminal maturation of blood monocytes (RIG) in vitro and in vivo into macrophages (MAC) occurs as a result of interactions with various cell types, To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured hunan MO with lymphocytes and/or with platelets. We found that intact platelets strongly promoted MO maturation under serum-free conditions as evident from the expression of differentiation-dependent antigens and morphology. To further characterize the differentiation-inducing component(s) we prepared membrane and cytosol fractions of platelets. Both fractions could induce MO maturation, comparable to intact platelets. Further centrifugation of the cytosolic fraction revealed that only the pellet of ultracentrifugation, e.g., membrane fragments, could induce MO differentiation, Digestion with either trypsin or neuraminidase could only partially inhibit this effect. The same was true for heat-treated fractions, indicating that this platelet-derived differentiation stimulus is not solely an intact protein, Next we prepared protein and lipid fractions of platelets. Treatment of MO with platelet proteins or platelet lipids clearly showed that only the lipid components were able to induce MO maturation. We propose components present in the lipid fraction of platelet membranes as possible inducers of MO maturation in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; HUMAN MACROPHAGE MATURATION; P-SELECTIN CD62; FC-GAMMA-RIII; ACTIVATED PLATELETS; MANNOSE RECEPTOR; EXPRESSION; NEUTROPHILS; INVITRO; PHENOTYPE; macrophages; mononuclear phagocytes; monocyte-platelet interaction; monocyte-lymphocyte interaction; platelet lipids |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2023 07:01 |
| Last Modified: | 12 Sep 2023 07:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49979 |
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