Jensen, Boye L. and Gambaryan, Stepan and Scholz, Holger C. and Kurtz, Armin (1998) K-ATP channels are not essential for pressure-dependent control of renin secretion. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 435 (5). pp. 670-677. ISSN 0031-6768, 1432-2013
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This study aimed to investigate the functional role of ATP-sensitive K+ (K+) channels in the control of renin secretion by renal perfusion pressure. We studied the effect of openers and blockers of K-ATP-channels on basal-and low-pressure-induced renin secretion from isolated perfused rat kidneys (IPRK). Cromakalim (0.1-10 mu M) stimulated basal renin secretion up to threefold and caused vasorelaxation in the IPRK. Both effects of cromakalim were attenuated by glibenclamide. Cromakalim stimulated renin secretion from isolated juxtaglomerular (JG) cells and from microdissected afferent arterioles, all of which suggests that K-ATP channel openers stimulate renin secretion at the level of JG cells. A de crease in the perfusion pressure from 13.3 to 9.33 kPa (100 mmHg to 70 mmHg) increased renin secretion twofold, and cromakalim further increased renin secretion. At 5.33 kPa (40 mmHg) renin secretion was increased sevenfold and was not further enhanced by cromakalim. The low-pressure-induced stimulation of renin secretion was not changed by glibenclamide. Finally, the dependence on calcium of the cromakalim-induced stimulation of renin was examined. Addition of the calcium antagonist amlodipine to the perfusate stimulated renin secretion, and in this situation cromakalim had no further effect. The stimulation of renin secretion by cromakalim in the IPRK was markedly attenuated by angiotensin II (1 nM). These results suggest that cromakalim could stimulate renin secretion through a pathway that includes a decrease in JG cell calcium. K-ATP channels are not essentially involved in pressure-sensitive renin secretion.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VASCULAR SMOOTH-MUSCLE; PERFUSED RAT-KIDNEY; JUXTAGLOMERULAR CELLS; CROMAKALIM; CALCIUM; ACTIVATION; OPENERS; STIMULATION; CHLORIDE; RABBIT; cromakalim; diazoxide; pinacidil; glibenclamide; afferent arteriole; juxtaglomerular |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Feb 2023 05:38 |
| Last Modified: | 28 Feb 2023 05:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49998 |
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