Grimm, Rudo and Singh, Hapreet and Rachel, Reinhard and Typke, Dieter and Zillig, Wolfram and Baumeister, Wolfgang (1998) Electron tomography of ice-embedded prokaryotic cells. BIOPHYSICAL JOURNAL, 74 (2). pp. 1031-1042. ISSN 0006-3495, 1542-0086
Full text not available from this repository.Abstract
Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm thick, and their structures were reconstructed to a resolution of 20-40 nm from tilt series comprising 50-140 images, The dose was kept within tolerable limits. A resolution of 20 nm allowed visualization of the individual stalks of the S-layer of Pyrobaculum aerophilum cells, which had undergone partial lysis, in three dimensions. The attainable resolution for low-dose electron tomography under different experimental conditions was theoretically investigated in terms of the specimen thickness. To obtain 2-nm resolution at 120 kV (300 kV), the specimen must not be thicker than 100 nm (150 nm). For a resolution of 10 nm, the maximum thickness is 450 nm (700 nm). An accelerating voltage of 300 kV is advantageous, mainly for specimens thicker than 100 nm. Experimental investigations so far have resulted in a resolution that is worse by a factor of 2-5 as compared to theory.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3-DIMENSIONAL STRUCTURE; BIOLOGICAL APPLICATIONS; MICROSCOPY; RECONSTRUCTION; SPECIMENS; SULFOLOBUS; VESICLES; ENVELOPE; FILTER; LAYER |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Feb 2023 09:28 |
| Last Modified: | 28 Feb 2023 09:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50094 |
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