Molitor, Michael and Dahl, Christiane and Molitor, Ilka and Schäfer, Ulrike and Speich, Norbert and Huber, Robert and Deutzmann, Rainer and Trüper, Hans G. (1998) A dissimilatory sirohaem-sulfite-reductase-type protein from the hyperthermophilic archaeon Pyrobaculum islandicum. MICROBIOLOGY-SGM, 144 (2). pp. 529-541. ISSN 1350-0872, 1465-2080
Full text not available from this repository. (Request a copy)Abstract
A sulfite-reductase-type protein was purified from the hyperthermophilic crenarchaeote Pyrobaculum islandicum grown chemoorganoheterotrophically with thiosulfate as terminal electron acceptor. In common with dissimilatory sulfite reductases the protein has an alpha(2) beta(2) structure and contains high-spin sirohaem, non-haem iron and acid-labile sulfide. The oxidized protein exhibits absorption maxima at 280, 392, 578 and 710 nm with shoulders at 430 and 610 nm. The isoelectric point of pH 8.4 sets the protein apart from all dissimilatory sulfite reductases characterized thus far. The genes for the alpha- and beta-subunits (dsrA and dsrB) are contiguous in the order dsrAdsrB and most probably comprise an operon with the directly following dsrG and dsrC genes. dsrG and dsrC encode products which are homologous to eukaryotic glutathione S-transferases and the proposed gamma-subunit of Desulfovibrio vulgaris sulfite reductase, respectively, dsrA and dsrB encode 44.2 kDa and 41.2 kDa peptides which show significant similarity to the two homologous subunits DsrA and DsrB of dissimilatory sulfite reductases. Phylogenetic analyses indicate a common protogenotic origin of the P. islandicum protein and the dissimilatory sulfite reductases from sulfate-reducing and sulfide-oxidizing prokaryotes. However, the protein from P. islandicum and the sulfite reductases from sulfate-reducers and from sulfur-oxidizers most probably evolved into three independent lineages prior to divergence of archaea and bacteria.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DESULFOVIBRIO-VULGARIS HILDENBOROUGH; GLUTATHIONE-S-TRANSFERASE; SPINACH NITRITE REDUCTASE; PYROCOCCUS-FURIOSUS; SULFATE REDUCTION; 3-DIMENSIONAL STRUCTURE; ASSIMILATORY NITRATE; CATALYTIC PROPERTIES; ELEMENTAL SULFUR; GEN-NOV; Pyrobaculum islandicum; hyperthermophilic archaeon; dissimilatory sirohaem sulfite reductase; nucleotide sequence; evolution |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Feb 2023 10:04 |
| Last Modified: | 28 Feb 2023 10:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50131 |
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