Haller, Katrin and Fabry, Stefan (1998) Brefeldin A affects synthesis and integrity of a eukaryotic flagellum. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 242 (3). pp. 597-601. ISSN 0006-291X, 1090-2104
Full text not available from this repository.Abstract
Eukaryotic flagella and cilia are highly dynamic organelles. In green algae like Chlamydomonas reinhardtii, flagella absorption and resynthesis is a normal process during the vegetative cell cycle. Rapid regeneration also occurs after stress-induced shedding of flagella. Ca2+ ions, protein synthesis, and a kinase activity are the main factors known to affect resynthesis. Recently, we have detected that certain small G proteins (Ypt/Rab) and a GTPase regulator (GDP dissociation inhibitor), known as regulatory elements of intracellular vesicle transport, are present in flagellar membranes of green algae, raising the possibility that the organelle's synthesis and/or integrity depends on functional membrane traffic. In this study, we examined the effect of brefeldin A (BFA), an inhibitor of intracellular membrane flow and Golgi function in animal and plant cells, on flagella regeneration in the colonial green alga Gonium pectorale. We show that low BFA concentrations (<1 mu g/ml) inhibit flagella outgrowth, while higher amounts cause dose-dependent deflagellation and cell death. Our findings provide experimental evidence for a direct connection between intracellular transport and eukaryotic flagella synthesis. (C) 1998 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHLAMYDOMONAS-REINHARDTII; GOLGI-APPARATUS; CELL BODY; MEMBRANE; PROTEINS; REGENERATION; ALGAE; TRANSPORT; DYNAMICS; TUBULIN |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Feb 2023 10:34 |
| Last Modified: | 28 Feb 2023 10:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50149 |
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