Svejgaard, Benjamin and Wehland, Markus and Ma, Xiao and Kopp, Sascha and Sahana, Jayashree and Warnke, Elisabeth and Aleshcheva, Ganna and Hemmersbach, Ruth and Hauslage, Jens and Grosse, Jirka and Bauer, Johann and Corydon, Thomas Juhl and Islam, Tawhidul and Infanger, Manfred and Grimm, Daniela (2015) Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat. PLOS ONE, 10 (8): e0135157. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
In this study we focused on gravity-sensitive proteins of two human thyroid cancer cell lines (ML-1; RO82-W-1), which were exposed to a 2D clinostat (CLINO), a random positioning machine (RPM) and to normal 1g-conditions. After a three (3d)-or seven-day-culture (7d) on the two devices, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1g-control cultures only formed adherent monolayers, unless the bottom of the culture dish was covered by agarose. In this case, the cytokines IL-6 and IL-8 facilitated the formation of MCS in both cell lines using the liquid-overlay technique at 1g. ML-1 cells grown on the RPM or the CLINO released amounts of IL-6 and MCP-1 into the supernatant, which were significantly elevated as compared to 1g-controls. Release of IL-4, IL-7, IL-8, IL-17, eotaxin-1 and VEGF increased time-dependently, but was not significantly influenced by the gravity conditions. After 3d on the RPM or the CLINO, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines. IL-6 and IL-8 stimulation of ML-1 cells for 3d and 7d influenced the protein contents of beta(1)-integrin, talin-1, Ki-67, and beta-actin dose-dependently in adherent cells. The beta(1)-integrin content was significantly decreased in AD and MCS samples compared with 1g, while talin-1 was higher expressed in MCS than AD populations. The proliferation marker Ki-67 was elevated in AD samples compared with 1g and MCS samples. The beta-actin content of R082-W-1 cells remained unchanged. ML-1 cells exhibited no change in beta-actin in RPM cultures, but a reduction in CLINO samples. Thus, we concluded that simulated microgravity influences the release of cytokines in follicular thyroid cancer cells, and the production of beta(1)-integrin and talin-1 and predicts an identical effect under real microgravity conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PAPILLARY THYROID-CARCINOMA; PARABOLIC FLIGHT MANEUVERS; ENDOTHELIAL GROWTH-FACTOR; DIFFERENTIAL GENE-EXPRESSION; ALTERED GRAVITY CONDITIONS; SIMULATED MICROGRAVITY; SPHEROID FORMATION; HUMAN CHONDROCYTES; INCREASES APOPTOSIS; COLORECTAL-CANCER; |
| Divisions: | Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Jun 2019 11:13 |
| Last Modified: | 14 Jun 2019 11:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5021 |
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