Huber, Robert and Dyba, Diana and Huber, Harald and Burggraf, Siegfried and Rachel, Reinhard (1998) Sulfur-inhibited Thermosphaera aggregans sp. nov., a new genus of hyperthermophilic archaea isolated after its prediction from environmentally derived 16S rRNA sequences. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 48. pp. 31-38. ISSN 0020-7713,
Full text not available from this repository. (Request a copy)Abstract
Recently, a new procedure was developed which allowed for the first time the isolation of a hyperthermophilic archaeum tracked by 16S rRNA analysis from a terrestrial hot solfataric spring ('Obsidian Pool', Yellowstone National Park, WY, USA). This novel isolate is characterized here. Cells are round cocci with a diameter of 0.2-0.8 mu m, occurring singly, in pairs, short chains and in grape-like aggregates. The aggregates exhibit a weak bluish-green fluorescence under UV radiation at 420nm. The new isolate is an anaerobic obligate heterotroph, using preferentially yeast extract for growth. The metabolic products include CO2, H-2, acetate and isovalerate. Growth is observed between 65 and 90 degrees C (optimum: 85 degrees C), from ph 5.0 to 7.0 (optimum: 6.5) and up to 0.7 % NaCl. The apparent activation energy for growth is about 149 kJ mol(-1). Elemental sulfur or hydrogen inhibits growth. The core lipids consist mainly of acyclic and cyclic glycerol diphytanyl tetraethers. The cell envelope contains a cytoplasmic membrane covered by an amorphous layer of unknown composition; there is no evidence for a regularly arrayed surface-layer protein. The G+C content is 46 mol%. On the basis of 16S rRNA sequence comparisons in combination with morphological. physiological and biochemical properties, the isolate represents a new genus within the Desulfurococcaceae, which has been named Thermosphaera. The type species is Thermosphaera aggregans, the type strain is isolate M11TL(T)(= DSM 11486(T)).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SULFATE-REDUCING BACTERIA; DEOXYRIBONUCLEIC-ACID; ENZYMATIC AMPLIFICATION; METHANOGENIC BACTERIA; OXIDIZING BACTERIA; ESCHERICHIA-COLI; SURFACE PROTEIN; ARCHAEBACTERIA; REPRESENTS; DESULFUROCOCCUS; Thermosphaera aggregans sp. nov.; archaea; hyperthermophile; 16S rRNA sequences |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Oct 2023 09:41 |
| Last Modified: | 31 Oct 2023 09:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50218 |
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