Tsiotis, Georgios and Hager-Braun, Christine and Wolpensinger, Bettina and Engel, Andreas and Hauska, Günter (1997) Structural analysis of the photosynthetic reaction center from the green sulfur bacterium Chlorobium tepidum. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1322 (2-3). pp. 163-172. ISSN 0005-2728, 0006-3002
Full text not available from this repository.Abstract
The reaction center (RC) core complex was isolated from the green sulfur bacterium Chlorobium tepidum and characterized by gel electrophoresis, gel filtration, and analytical ultracentrifugation. The purified complex contained the PscA and PscC subunits and a small amount of the Fenna-Matthews-Olson protein (FMO protein) as an impurity. The mass of the core complexes was found to be 248kDa by scanning transmission electron microscopy (STEM). This is compatible with the presence of two copies of the PscA subunit and at least one copy of the PscC subunit, and provides evidence that the isolated complex exists as a monomer. Digital images of negatively stained RC complexes were recorded by STEM and analyzed by single-particle averaging, The complex had a length of 14 nm and a width of 8 nm, comparable to the length and width of the monomeric cyanobacterial PSI complex. The averages revealed a pseudo two-fold symmetry axis, which is a prominent structural element of the monomeric form. (C) 1997 Elsevier Science B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | I REACTION-CENTER; REACTION-CENTER COMPLEX; CYANOBACTERIUM SYNECHOCOCCUS-SP; LIMICOLA F THIOSULFATOPHILUM; PHOTOSYSTEM-I; RHODOPSEUDOMONAS-VIRIDIS; 3-DIMENSIONAL STRUCTURE; 2-DIMENSIONAL CRYSTALS; ELECTRON-MICROSCOPY; PROTEIN SUBUNITS; electron microscopy; green sulfur bacteria; reaction center |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Günter Hauska |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2023 11:26 |
| Last Modified: | 01 Mar 2023 11:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50330 |
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