Schmiedeknecht, Gerno and Büchler, Christa and Schmitz, Gerd (1997) A bidirectional promoter connects the p14.5 gene to the gene for RNase P and RNase MRP protein subunit hPOP1. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 241 (1). pp. 59-67. ISSN 0006-291X, 1090-2104
Full text not available from this repository.Abstract
We have identified the functional promoter of the translational inhibitor p14.5, the human homologue to a rat perchloric acid-soluble protein (PSP), a mouse heat-responsive protein (Hrp12) and a goat tumor antigen (UK114). Sequence analysis revealed a GC-rich promoter with several consensus sequences for transcription factors, but no TATA- and CAAT-box. To confirm promoter activity, DNA fragments of the p14.5 5'-flanking region were ligated in front of the luciferase gene and were transfected into HeLa and HepG2 cells. A minimal promoter between nt -104 and nt +88 relative to the transcription start site was responsible for basal activity. Furthermore, we observed a head-to-head orientation of p14.5 to the gene for the protein subunit of RNase P and MRP ribonucleoproteins (hPOP1). Luciferase assays with fragments of the hPOP1 5'-flanking region revealed a minimal promoter between nt -20 and nt +98 relative to the start of transcription. These data indicate that the 102 bp region between p14.5 and hPOP1 can act as a bidirectional promoter. The p14.5-hPOP1-cluster was mapped to chromosome 8q22 using in situ hybridization technique. (C) 1997 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EUKARYOTIC INITIATION FACTOR-2-ALPHA; HEAD-TO-HEAD; TRANSCRIPTION FACTOR; INSITU HYBRIDIZATION; EXPRESSION; ACID; ANTIGEN; CELLS; TUMOR; SEQUENCE |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2023 05:07 |
| Last Modified: | 28 Mar 2023 05:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50337 |
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