Luppa, Peter and Brückner, Christine and Schwab, Ingrid and Hauck, Sabine and Schmidmayr, Stefan and Birkmayer, Christian and Paulus, Birgit and Hauptmann, Hagen (1997) 7 alpha-Biotinylated testosterone derivatives as tracers for a competitive chemiluminescence immunoassay of testosterone in serum. CLINICAL CHEMISTRY, 43 (12). pp. 2345-2352. ISSN 0009-9147, 1530-8561
Full text not available from this repository.Abstract
Ring core-biotinylated testosterone tracers were synthesized with bridges of three different lengths connecting the biotin moiety to the steroid core (7 alpha-C-n-Bio-T, n = 3, 6, or 11). Together with a position 7-specific polyclonal anti-testosterone antibody, we used the 7 alpha-C-11-Bio-T tracer to develop a novel, labeled-hapten competitive immunoassay for total testosterone in serum. (The C-3 and C-6 tracers proved to be not suitable for analogous immunoassays.) Enhanced chemiluminescence signal was generated by use of a second immobilized antibody and a streptavidin-horseradish peroxidase conjugate. The measuring range of the assay is 0.2-20.0 nmol/L, linearity of serial dilutions can be demonstrated, the lower detection limit is 0.125 nmol/L, and the interassay imprecisions are 13-16%. Accuracy determinations in mass spectrometry-controlled reference specimens showed a mean recovery of 95%. In addition, the assay shows low cross-reactivities, demonstrating the favorable specificity of the combination of a "nearly native" tracer with a position analog antibody. The optimized steric structure and the long spacer arm of the biotinylated testosterone tracer make this chemiluminescence assay well-suited for measuring total testosterone concentration in serum.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHROMATOGRAPHY MASS-SPECTROMETRY; CLINICAL-CHEMISTRY; ESTRADIOL; ESTRONE; ASSAY |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2023 05:42 |
| Last Modified: | 28 Mar 2023 05:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50350 |
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