Kröger, Nils and Lehmann, Gerhard and Rachel, Reinhard and Sumper, Manfred (1997) Characterization of a 200-kDa diatom protein that is specifically associated with a silica-based substructure of the cell wall. EUROPEAN JOURNAL OF BIOCHEMISTRY, 250 (1). pp. 99-105. ISSN 0014-2956
Full text not available from this repository.Abstract
The cell wall of a diatom is made up of a silica-based scaffold and organic macromolecules. Proteins located in the cell wall are believed to control morphogenesis of the species-specific silica structures of the scaffold. However, data that correlate distinct silica elements and specific proteins within the diatom cell wall have not been reported. Here, the cell wall protein HEP2OO (200-kDa HE-extractable protein) from the diatom Cylindrotheca fusiformis is identified and characterized. HEP200 is tightly associated with a substructure of the silica scaffold. It is a member of a new protein family, of which two more members are identified. Each member displays the same bipartite structure. The N-terminal part consists of a variable number of a repeated sequence motif (PSCD domain), whereas the C-terminal part is unique, Immunolocalization experiments revealed the arrangement of different proteins within the cell wall. Frustulins, a previously described group of glycoproteins, constitute the outer coat of the cell wall and exhibit a ubiquitous distribution. In contrast, HEP200 is specifically located at a subset of about six silica strips in intact cell walls, shielded by frustulins. This study therefore identifies a diatom cell wall protein (HEP200) that is associated with a distinct substructure of the silica scaffold.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VOLVOX-CARTERI; GLYCOPROTEINS; biomineralization; Cylindrotheca fusiformis; girdle band; immunolocalization; morphogenesis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Alumni or Retired > Prof. Dr. Manfred Sumper Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Mar 2023 07:34 |
| Last Modified: | 02 Mar 2023 07:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50408 |
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