Kuntz, Cornelia and Sonnenbichler, Johann and Sonnenbichler, Isolde and Sumper, Manfred and Zeitler, Reinhard (1997) Isolation and characterization of dolichol-linked oligosaccharides from Haloferax volcanii. GLYCOBIOLOGY, 7 (7). pp. 897-904. ISSN 0959-6658, 1460-2423
Full text not available from this repository.Abstract
Biosynthesis of procaryotic glycoproteins has been studied in some detail only in the extreme halophile Halobacterium halobium. To extend these studies for a moderate halophile, dolichol phosphate-linked oligosaccharides were isolated and characterized from Haloferax volcanii, Mannosyl (beta 1-->4)galactosyl phosphodolichol could be characterized as a main component by GC/MS permethylation analysis, mass spectrometry and H-1-NMR-spectroscopy, Furthermore, two additional components, present in lower quantities, were partially characterized and identified as a tetrasaccharyl phosphodolichol containing mannose, galactose, and rhamnose in the ratio of 2:1:1 as well as another dihexosyl phosphodolichol. Both the latter compounds contain an additional charged group, All these oligosaccharides were found to be linked to a dolichol consisting of 11 or 12 isoprene units including a saturated omega-terminal isoprene residue.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHYLATION ANALYSIS; GLYCOPROTEINS; GLYCOSYLATION; BIOSYNTHESIS; IDENTIFICATION; HALOBACTERIUM; PURIFICATION; GLYCOLIPIDS; MODERATE; PROTEIN; dolichol; glycosylation; halobacteria; Haloferax volcanii; oligosaccharides |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Alumni or Retired > Prof. Dr. Manfred Sumper |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2023 07:46 |
| Last Modified: | 28 Mar 2023 07:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50506 |
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