MullerLadner, U and Elices, MJ and Kriegsmann, JB and Strahl, D and Gay, RE and Firestein, GS and Gay, S (1997) Alternatively spliced CS-1 fibronectin isoform and its receptor VLA-4 in rheumatoid arthritis synovium. JOURNAL OF RHEUMATOLOGY, 24 (10). pp. 1873-1880. ISSN 0315-162X,
Full text not available from this repository.Abstract
Objective. Extracellular matrix components and cell adhesion molecules play a role in the pathogenesis of rheumatoid arthritis (RA), Interaction between the integrin very late antigen-4 (VLA-4) and the connecting segment-1 (CS-1) fibronectin (FN) isoform may contribute to lymphocyte interaction in RA synovium. We examined both mRNA and protein expression of CS-I FN in inflamed synovium, and VLA-4 expression in synovial ! tissue and on cultured fibroblast-like synoviocytes from patients with RA. Methods, Snap frozen synovial tissue specimens of 10 patients with RA and 4 patients with osteoarthritis were examined for expression of CS-I FN mRNA and protein by ill situ hybridization and immunohistochemistry. VLA-4 expression of synovial fibroblasts and in synovial tissue was evaluated by flow cytometry and immunohistochemistry. Results, CS-1 FN mRNA was detected in RA lining layer synoviocytes, around terminal vessels, and in endothelial cells. Double labeling revealed that most lining synoviocytes expressing CS-I FN mRNA were CD68 negative, VLA-4 was found in RA synovial fibroblasts, sublining mononuclear cells, and lymphoid aggregates. Conclusion. Our findings suggest that expression of CS-1 FN may partially correlate with cell proliferation in the RA lining layer. VLA-4 was found in RA synovial lining, as well as on cultured synovial fibroblasts, Thus, VLA-4/CS-1 FN interaction may facilitate lymphocyte interaction and synovial proliferation in RA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-ADHESION MOLECULE-1; FIBROBLAST-LIKE SYNOVIOCYTES; TUMOR-NECROSIS-FACTOR; MESSENGER-RNA; LEUKOCYTE ADHESION; INTEGRIN ALPHA-4-BETA-1; PLASMA FIBRONECTIN; CS1 FIBRONECTIN; EXPRESSION; BINDING; rheumatoid arthritis; connecting segment-1; fibronectin; very late antigen-4; fibroblasts; macrophages |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50530 |
Actions (login required)
 |
View Item |
