Rogler, G and Andus, T and Aschenbrenner, E and Vogl, D and Falk, W and Scholmerich, J and Gross, V (1997) Alterations of the phenotype of colonic macrophages in inflammatory bowel disease. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 9 (9). pp. 893-899. ISSN 0954-691X,
Full text not available from this repository.Abstract
Background: Intestinal macrophages play an important role in mucosal inflammation. In normal colonic mucosa we recently demonstrated a unique macrophage phenotype with attenuated immune functions. Here we present an analysis of the alterations of the phenotype of colonic macrophages in inflammatory bowel disease (IBD). Methods: Intestinal macrophages were isolated from biopsies of patients with IBD (n = 20). Flow cytometric triple fluorescence analysis was applied to study CD14, CD16, CD33, HLA-DR, CD44, CD11b, CD11c and CD3/CD19 expression. Results: In IBD there was an increase in expression not only of CD14 compared to control mucosa (36.0% +/- 13.2% vs. 10.5% +/- 3.8%, P< 0.0001) but also of CD16 (28.6% +/- 10.3% vs. 10.1% +/- 3.9%, P< 0.0001), HLA-DR (53.1% +/- 15.9% vs. 27.3% +/- 9.2%, P< 0.0005), CD11b (42.8% +/- 14.2% vs. 17.4% +/- 6.8%, P< 0.0001) and CD11c (35.1% +/- 15.9% vs. 17.8% +/- 10.4%, P< 0.005.). Furthermore, a hitherto undescribed new population of macrophages could be detected by flow cytometry only in patients with ulcerative colitis (CD16(++), CD11b(++), CD14(low), CD33(low), CD11c(-)) accounting for 5.8% of all cells isolated. Conclusion: In contrast to colonic macrophages from normal mucosa, there is a significantly higher expression of CD14, CD16, HLA-DR, CD11b and CD11c in IBD, indicating additional macrophage populations in the inflamed mucosa. This may reflect either a recruitment of new cells from the circulation or a change in phenotype of resident cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERIPHERAL-BLOOD MONOCYTES; ALVEOLAR MACROPHAGES; PULMONARY SARCOIDOSIS; FLOW-CYTOMETRY; LAMINA PROPRIA; CROHNS-DISEASE; EXPRESSION; RECRUITMENT; RECEPTOR; MUCOSA; colonic macrophages; FACS analysis; inflammatory bowel disease; Crohn's disease; ulcerative colitis; CD14; CD33 |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50598 |
Actions (login required)
 |
View Item |
