Maack, S and Knuechel, R and Hofstaedter, F and Stark, A and Schlegel, J (1997) Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA. INTERNATIONAL JOURNAL OF ONCOLOGY, 11 (2). pp. 383-387. ISSN 1019-6439,
Full text not available from this repository.Abstract
The aim of the present study was to detect differentially expressed genes in human bladder cancer cell lines using a non-radioactive RNA fingerprinting technique (arbitrarily primed polymerase chain reaction of RNA, RAP-PCR). The two clonal urothelial cancer cell lines, RT4 and J82, show different growth kinetics upon stimulation with EGF. By RAP-PCR we detected changes in band patterns for J82 cells treated with EGF but not for RT4 cells. Polymorphic fragments were further characterized and sequences from two of these gave a perfect match to the coding sequence of the human tropomyosin gene TM30(pl) and the human MAC30 gene, respectively. In accordance with the results of RAP-PCR downregulation in EGF-stimulated J82 cells could be demonstrated by reverse transcription PCR.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPIDERMAL GROWTH-FACTOR; CARCINOMA; LINES; TROPOMYOSIN; RECEPTORS; DISPLAY; bladder cancer; transcription; tropomyosin; arbitrarily primed PCR |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50669 |
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