Pietzsch, A and Buchler, C and Aslanidis, C and Schmitz, G (1997) Identification and characterization of a novel monocyte/macrophage differentiation-dependent gene that is responsive to lipopolysaccharide, ceramide, and lysophosphatidylcholine. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 235 (1). pp. 4-9. ISSN 0006-291X,
Full text not available from this repository.Abstract
A novel differentiation-dependent cDNA (DIF-2) has been isolated from human mononuclear phagocytes by differential display. The full-length cDNA was cloned and sequenced. DIF-2 consists of 156 amino acids and has a predicted isoelectric point of 8.84. The mRNA is expressed in freshly isolated monocytes and is down-regulated significantly when monocytes are subjected to differentiation. A similar differentiation-dependent downregulation is observed in normal hepatocytes compared to undifferentiated HepG2 cells. The mRNA expression in monocytes is sensitive to lipopolysaccharide and ceramide which both strongly increase DIF-2 transcription, while lysophosphatidylcholine results in a weaker upregulation of DIF-2 expression. A DIF-2 homologous gene has been previously isolated from mouse fibroblasts and was shown to be a serum growth factor-inducible immediate early gene. Our results indicate that DIF-2 represents a gene which is regulated in differentiation processes and strongly responsive to lipopolysaccharide, ceramide and lysophosphatidylcholine. (C) 1997 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CULTURED HUMAN; MESSENGER-RNA; CELLS; MACROPHAGES; MONOCYTES; PROTEIN; DISPLAY; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50761 |
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