Van Landuyt, Kirsten L. and Krifka, Stephanie and Hiller, Karl-Anton and Bolay, Carola and Waha, Claudia and Van Meerbeek, Bart and Schmalz, Gottfried and Schweikl, Helmut (2015) Evaluation of cell responses toward adhesives with different photoinitiating systems. DENTAL MATERIALS, 31 (8). pp. 916-927. ISSN 0109-5641, 1879-0097
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Objectives. The photoinitiator diphenyl-(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) is more reactive than a camphorquinone/amine (CQ) system, and TPO-based adhesives obtained a higher degree of conversion (DC) with fewer leached monomers. The hypothesis tested here is that a TPO-based adhesive is less toxic than a CQ-based adhesive. Methods. A CQ-based adhesive (SBU-CQ) (Scotchbond Universal, 3M ESPE) and its experimental counterpart with TPO (SBU-TPO) were tested for cytotoxicity in human pulp-derived cells (tHPC). Oxidative stress was analyzed by the generation of reactive oxygen species (ROS) and by the expression of antioxidant enzymes. A dentin barrier test (DBT) was used to evaluate cell viability in simulated clinical circumstances. Results. Unpolymerized SBU-TPO was significantly more toxic than SBU-CQ after a 24h exposure, and TPO alone (EC50 = 0.06 mM) was more cytotoxic than CQ (EC50 = 0.88 mM), EDMAB (EC50 = 0.68 mM) or CQ/EDMAB (EC50 = 0.50 mM). Cultures preincubated with BSO (L-buthionine sulfoximine), an inhibitor of glutathione synthesis, indicated a minor role of glutathione in cytotoxic responses toward the adhesives. Although the generation of ROS was not detected, a differential expression of enzymatic antioxidants revealed that cells exposed to unpolymerized SBU-TPO or SBU-CQ are subject to oxidative stress. Polymerized SBU-TPO was more cytotoxic than SBU-CQ under specific experimental conditions only, but no cytotoxicity was detected in a DBT with a 200 mu m dentin barrier. Significance. Not only DC and monomer-release determine the biocompatibility of adhesives, but also the cytotoxicity of the (photo-)initiator should be taken into account. Addition of TPO rendered a universal adhesive more toxic compared to CQ; however, this effect could be annulled by a thin dentin barrier. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PULP-DERIVED CELLS; OXIDATIVE STRESS; DENTAL ADHESIVES; DIFFERENT INITIATORS; ELUTABLE SUBSTANCES; INDUCED APOPTOSIS; COMPOSITE RESINS; MONOMER ELUTION; CONVERSION; CYTOTOXICITY; Dental adhesive; Photoinitiator; TPO; CQ; Cytotoxicity; Reactive oxygen species; Apoptosis; Biocompatibility |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Jun 2019 11:55 |
| Last Modified: | 18 Jun 2019 11:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5078 |
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