Weiss, Thomas and Bernhardt, Günther and Buschauer, Armin and Jauch, Karl-Walter and Zirngibl, Hubert (1997) High-resolution reversed-phase high-performance liquid chromatography analysis of polyamines and their monoacetyl conjugates by fluorescence detection after derivatization with N-hydroxysuccinimidyl 6-quinolinyl carbamate. ANALYTICAL BIOCHEMISTRY, 247 (2). pp. 294-304. ISSN 0003-2697, 1096-0309
Full text not available from this repository.Abstract
A highly sensitive, accurate, and reproducible HPLC method for the determination of all natural polyamines and their monoacetyl conjugates is described. The presented method is based on precolumn derivatization with N-hydroxysuccinimidyl 6-quinolinyl carbamate (HSQC) followed by C-18-HPLC separation using a ternary gradient and fluorescence detection (lambda(Ex) = 248 nm, lambda(Em) = 398 nm). The derivatives of the four main polyamines (putrescine, cadaverine, spermidine, and spermine) and the internal standard were synthesized on a preparative scale and characterized, especially with respect to their molar absorptivities and fluorescence quantum yields. The limits of detection of the highly stable derivatives ranged from 30 to 130 fmol (injection volume 10 mu l) for putrescine and N-acetylcadaverine, respectively (signal to noise ratio = 10), with excellent Linearity within the range hom 1 to 100 mu M. High reproducibility for both retention times and peak areas, with coefficients of variation ranging from 0.14 to 0.88% and from 1.83 to 3.80%, respectively, were achieved. Provided that deproteinization of the samples was carried out immediately, recoveries of the analytes from homogenates of pancreatic cancer xenografts were high and reproducible. The optimized method was applied to the determination of the polyamine con tent of cultured pancreatic cancer cells and of tissue from colorectal adenocarcinoma, proving precise and reproducible quantification in these complex biological matrices. (C) 1997 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMPROVED SEPARATION SYSTEMS; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE; AMINO-ACIDS; DERIVATIVES; METABOLISM; URINE; TISSUE; GROWTH; N-1,N-8-DIACETYLSPERMIDINE; N-1,N-12-DIACETYLSPERMINE |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 May 2023 06:29 |
| Last Modified: | 09 May 2023 06:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50831 |
Actions (login required)
 |
View Item |
