Muckenschnabel, I. and Bernhardt, Günther and Spruss, Thilo and Buschauer, Armin (1997) A versatile high-performance liquid chromatography method for the measurement of melphalan tailored to the optimization of hyperthermic isolated limb perfusion. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 5 (3). pp. 129-137. ISSN 0928-0987, 1879-0720
Full text not available from this repository.Abstract
A simple isocratic RP-18 ion-pair high-performance liquid chromatography method with fluorimetric detection and N-phenyl-2,2'-iminodiethanol as internal standard is presented for the determination of melphalan (the standard cytostatic in isolated limb perfusion (ILP) of patients with malignant melanoma) from aqueous solutions (e.g. cell culture medium or perfusate) and from biological material (melanoma cells, blood, muscle, skin, blood vessel, nerve). In aqueous solutions both hydrolysis products of melphalan were also quantified with high sensitivity, which allowed the measurement of the hydrolysis kinetics in H2O, 0.9% NaCl solution and cell culture medium at 37, 41.5 and 43 degrees C. Despite its reduced half life at elevated temperatures, melphalan accumulation by SK-MEL-24 cells was significantly higher at 41.5 degrees C than at 37 degrees C. With optimal solid-phase extraction, 2.6 ng melphalan and 45 ng internal standard were quantified (signal to noise ratio of 10:1) with high precision in less than 50 mg of tissue, collected from a rat after experimental ILP.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REGIONAL ISOLATED PERFUSION; L-PHENYLALANINE MUSTARD; FLUORESCENCE DETECTION; HYDROLYSIS PRODUCTS; PLASMA MELPHALAN; MELANOMA; DERIVATIZATION; TISSUE; melphalan; HPLC; hydrolysis kinetics; cellular uptake; isolated limb perfusion |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 May 2023 06:56 |
| Last Modified: | 09 May 2023 06:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50843 |
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