Sandner, Peter and Wolf, Konrad and Bergmaier, Ulrike and Gess, Bernhard and Kurtz, Armin (1997) Hypoxia and cobalt stimulate vascular endothelial growth factor receptor gene expression in rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 433 (6). pp. 803-808. ISSN 0031-6768, 1432-2013
Full text not available from this repository.Abstract
This study aimed to examine the influence of acute tissue hypo-oxygenation on the expression of the vascular endothelial growth factor (VEGF) receptor genes. To this end male Sprague-Dawley rats were exposed to different hypoxic conditions such as 10% or 8% oxygen, 0.1% carbon monoxide and cobalt chloride (60 mg/kg) for 6 h and the abundance of flt-1, flt-4 and flk-1 mRNA in lungs and livers was determined by RNase protection assay. The relative proportions of flt-1, flt-4 and flk-1 were 10 : 2.5 : 1 and 10 : 10 : 2 in normoxic lungs and livers, respectively. It was found that 8% but not 10% oxygen increased flt-1 mRNA two- to threefold in both organs, whilst flt-4 and flk-1 mRNA were not changed by acute inspiratory hypoxia. Carbon monoxide inhalation also increased flt-1 mRNA but not flt-4 or flk-1 mRNA in both organs. Subcutaneous cobalt administration increased flt-1 mRNA in the livers only, whilst flt-4 and flk-1 mRNA remained unchanged. These findings show that acute tissue hypo-oxygenation is a rather selective stimulus for flt-1 gene expression. The efficiency of the different manoeuvres applied to stimulate flt-1 gene expression is rather similar to the stimulation of erythropoietin gene expression. It is not unreasonable to assume, therefore, that the oxygen-dependent regulation of both genes at the cellular level has significant similarities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ERYTHROPOIETIN MESSENGER-RNA; TYROSINE KINASE; SIGNAL-TRANSDUCTION; TUMOR ANGIOGENESIS; MUSCLE CELLS; MODULATION; VEGF; FLT4; KDR; INDUCTION; vascular endothelial growth factor; tyrosine kinase; flt-1; flt-4; flk-1; erythropoietin |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2023 09:32 |
| Last Modified: | 11 May 2023 09:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50960 |
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