Gess, Bernhard and Wolf, Konrad and Kurtz, Armin (1997) Lack of control by immediate early response genes in the oxygen regulation of erythropoietin gene expression. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 433 (6). pp. 827-831. ISSN 0031-6768, 1432-2013
Full text not available from this repository.Abstract
This study sought to investigate the role of immediate early genes in the stimulation of erythropoietin (EPO) gene expression by hypoxia. To this end freshly isolated rat hepatocytes were exposed to either normoxia (20% oxygen) or to hypoxia (1% oxygen) and the mRNA levels of the early genes c-fos, c-jun, c-myc and EGR-1 were monitored together with EPO mRNA. Isolation of the cells from the livers strongly stimulated the expression of c-fos, c-jun, c-myc and of EGR-1, whilst EPO gene expression remained unchanged. Exposure of the isolated hepatocytes to hypoxia did not further change early gene expression when compared with cells kept under normoxic conditions. EPO mRNA increased time dependently with a delay of 1 h after onset of hypoxia. These findings suggest that even strong activation of early gene expression has no influence on EPO gene expression, whilst activation of EPO gene expression during hypoxia can happen without change of early gene expression. It appears, therefore, as if immediate early genes are not causally involved in the sequence of events by which hypoxia stimulates EPO gene expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDUCIBLE FACTOR-I; MESSENGER-RNA; C-FOS; HYPOXIA; PROTEIN; RAT; TRANSCRIPTION; INDUCTION; BINDING; ACCUMULATION; erythropoietin; hypoxia; immediate early genes; rat hepatocytes |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2023 09:36 |
| Last Modified: | 11 May 2023 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50962 |
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