Beck, Petra and Huber, Robert (1997) Detection of cell viability in cultures of hyperthermophiles. FEMS MICROBIOLOGY LETTERS, 148 (1). pp. 11-14. ISSN 0378-1097, 1574-6968
Full text not available from this repository.Abstract
Fluorescent dyes were assessed with regard to their ability to discriminate between viable and non-viable cells of hyperthermophilic archaea and bacteria. Using bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC(4)(3)), a membrane potential-sensitive probe, a safe and rapid discrimination of viable cells was possible by fluorescence microscopy. Single viable individuals, identified by DiBAC(4)(3), were selectively isolated from mixtures of viable and dead cells by the use of a laser microscope ('optical tweezers') and grown in pure culture.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLOW-CYTOMETRY; BACTERIA; MICROORGANISMS; hyperthermophile; viability; esterase-substrate fluorescent dye; membrane potential-sensitive dye; fluorescence microscopy; laser microscope |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2023 10:17 |
| Last Modified: | 11 May 2023 10:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50994 |
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