Krause, SW and Kreutz, M and Andreesen, R (1997) Differential effects of cell adherence on LPS-stimulated cytokine production by human monocytes and macrophages. IMMUNOBIOLOGY, 196 (5). pp. 522-534. ISSN 0171-2985,
Full text not available from this repository.Abstract
It is well known that adherence of monocytes (MO) to extracellular matrix substrates or tissue culture plastic activates these cells and induces the expression of a multitude of genes. Especially, it was described, that MO are primed by cell adhesion to produce higher amounts of some cytokines, e.g. interleukin (IL)-8 and tumor necrosis factor alpha (TNF-alpha). In order to investigate adherence-induced effects upon cytokine production, we seeded MO into tissue cultures and stimulated cells by lipopolysaccharide (LPS) simultaneously or at later time points. An increasing time-lag between cell adhesion and LPS-stimulation led to differential effects upon cytokine production: whereas TNF was upregulated (in accordance with reports by others), granulocyte colony stimulating factor (G-CSF) was considerably down-regulated. In contrast, G-CSF production did not change, when cells were kept under non-adherent conditions in n hole blood. In adherent cultures down-regulation of G-CSF could already be observed after two hours with a maximum after 24 h and was paralleled by a much lower abundance of G-CSF mRNA. Adhesion induced a significant suppression of G-CSF comparable to MO, if mature macrophages derived from MO in vitro were examined. Furthermore, two other cytokines, granulocyte-macrophage (GM)-CSF and IL-6, were also down-regulated following adhesion. In conclusion, activation of mononuclear phagocytes by adhesion can Lead to ''priming'' for the ''silencing'' for the production of others.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR NECROSIS FACTOR; GENE-EXPRESSION; DEVELOPMENTAL REGULATION; MONOCLONAL-ANTIBODY; BLOOD MONOCYTES; TNF-ALPHA; GM-CSF; ADHESION; RECEPTOR; INVITRO; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50998 |
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