Sparrer, Helmut and Rutkat, Kerstin and Buchner, Johannes (1997) Catalysis of protein folding by symmetric chaperone complexes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 94 (4). pp. 1096-1100. ISSN 0027-8424
Full text not available from this repository.Abstract
The GroE chaperones of Escherichia coli assist protein folding under physiological and heat shock conditions in an ATP-dependent way. Although a number of details of assisted folding have been elucidated, the molecular mechanism of the GroE cycle remains unresolved, Were we present an experimental system that allows the direct analysis of the GroE-mediated folding cycle under stringent conditions. We demonstrate that the GroE proteins efficiently catalyze the folding of kinetically trapped folding intermediates of a mutant of maltose-binding protein (MBP Y283D) in an ATP-dependent way, GroES plays a kev role in this reaction cycle, accelerating the folding of the substrate protein MBP Y283D up to 50-fold. Interestingly, catalysis of the folding reaction requires the formation of symmetrical football-shaped GroEL . GroES(2) particles and the intermediate release of the nonnative protein from the chaperone complex. Our results show that, in the presence of GroES, the complex architecture of the GroEL toroids allows maintenance of two highly interregulated rings simultaneously active in protein folding.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROEL-GROES COMPLEXES; IN-VIVO; BINDING; CYCLE; AGGREGATION; POLYPEPTIDE; MECHANISM; RELEASE; ATP; GroE; maltose binding protein; molecular chaperones; chaperonins; heat shock |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2023 05:15 |
| Last Modified: | 17 May 2023 05:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51033 |
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