Blöchl, Elisabeth and Rachel, Reinhard and Burggraf, Siegfried and Hafenbradl, Doris and Jannasch, Holger W. and Stetter, Karl O. (1997) Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit for life to 113 degrees C. EXTREMOPHILES, 1 (1). pp. 14-21. ISSN 1431-0651, 1433-4909
Full text not available from this repository.Abstract
A novel, irregular, coccoid-shaped archaeum was isolated from a hydrothermally heated black smoker wall at the TAG site at the Mid Atlantic Ridge (depth 3650 meters). It grew at between 90 degrees C and 113 degrees C (optimum 106 degrees C) and pH 4.0-6.5 (optimum 5.5) and 1%-4% salt (optimum 1.7%). The organism was a facultatively aerobic obligate chemolithoautotroph gaining energy by H-2-oxidation. Nitrate, S2O32-, and low concentrations of O-2 (up to 0.3% v/v) served as electron accepters, yielding NH4+, H2S, and H,O as end products? respectively. Growth was inhibited by acetate, pyruvate, glucose, starch, or sulfur. The new isolate was able to form colonies on plates (at 102 degrees C) and to grow at a pressure of 25000kPa (250 bar). Exponentially growing cultures survived a one-hour autoclaving at 121 degrees C. The GC content was 53mol%. The core lipids consisted of glycerol - dialkyl glycerol tetraethers and traces of 2, 3-di- O-phytanyl-sn-glycerol. The cell wall was composed of a surface layer of tetrameric protein complexes arranged on a p4-lattice (center-to-center distance 18.5nm). By its 16S rRNA sequence, the new isolate belonged to the Pyrodictiaceae. Based on its GC-content, DNA homology, S-layer composition, and metabolism, we describe here a new genus: which we name Pyrolobus (the ''fire lobe''). The type species is Pyrolobus fumarii (type strain 1A; DSM 11204).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHANOPYRUS-KANDLERI; PYRODICTIUM-ABYSSI; ARCHAEBACTERIA; 110-DEGREES-C; LIPIDS; GROWTH; 105-DEGREES-C; METHANOGENS; ORGANISMS; TAXONOMY; archaea; hyperthermophilic; temperature; hydrothermal; taxonomy; Marine |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2023 05:59 |
| Last Modified: | 17 May 2023 05:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51053 |
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