Rubenwolf, S and Schutt, H and Nevels, M and Wolf, H and Dobner, T (1997) Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex. JOURNAL OF VIROLOGY, 71 (2). pp. 1115-1123. ISSN 0022-538X,
Full text not available from this repository.Abstract
The adenovirus type 5 (Ad5) early 1B (E1B) 55-kDa (E1B-55kDa)-E4orf6 protein complex has been implicated in the selective modulation of nucleocytoplasmic mRNA transport at late times after infection. Using a combined immunoprecipitation-immunoblotting assay, me mapped the domains in E1B-55kDa required for the interaction with the E4orf6 protein in lytically infected A549 cells. Several domains in the 496-residue 55-kDa polypeptide contributed to a stable association with the E4orf6 protein in E1B mutant virus-infected cells. Linker insertion mutations at amino acids 180 and 224 caused I educed binding of the E4orf6 protein, whereas linker insertion mutations at amino acid 143 and in the central domain of E1B-55kDa eliminated the binding of the E4orf6 protein. Earlier work showing that the central domain of E1B-55kDa is required for binding to p53 and the recent observation that the E4orf6 protein also interacts with the tumor suppressor protein led us to suspect that p53 might play a role in the E1B-E4 protein interaction. However, coimmunoprecipitation assays with extracts prepared from infected p53-negative H1299 cells established that p53 is not needed for the E1B-E4 protein interaction in adenovirus-infected cells. Using two different protein-protein interaction assays, me also mapped the region in the E4orf6 protein required for E1B-55kDa interaction to the amino-terminal 55 amino acid residues, Interestingly, both binding assays established that the same region in the E4orf6/7 protein can potentially interact with E1B-55kDa. Our results demonstrate that two distinct segments in the 55-kDa protein encoding the transformation and late lytic functions independently interact with p53 and the E4orf6 protein in vivo and provide further insight by which the multifunctional 55-kDa E1B protein can exert its multiple activities in lytically infected cells and in adenovirus transformation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRODUCTIVELY INFECTED-CELLS; LATE GENE-EXPRESSION; MESSENGER-RNA ACCUMULATION; EARLY REGION-4; TRANSFORMED-CELLS; DNA-REPLICATION; LYTIC INFECTION; TUMOR-ANTIGENS; P53; TRANSCRIPTION; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51076 |
Actions (login required)
 |
View Item |
