Hager-Braun, Christine and Jarosch, Ursula and Hauska, Günter and Nitschke, Wolfgang and Riedel, Astrid (1997) EPR studies of the terminal electron acceptors of the green sulfur bacterial reaction centre. Revisited. PHOTOSYNTHESIS RESEARCH, 51 (2). pp. 127-136. ISSN 0166-8595, 1573-5079
Full text not available from this repository.Abstract
The EPR spectroscopic and electrochemical properties of FeS centres in membrane fragments and in isolated reaction centres from green sulfur bacteria purified as published previously (Hager-Braun C, Xie D-L, Jarosch U, Herold E, Buttner M, Zimmermann R, Deutzmann R, Hauska G and Nelson N (1995) Biochemistry 34: 9617-9624) were compared for the species Chlorobium limicola and Chlorobium tepidum. In addition, membrane samples from a third green sulfur bacterium, Chlorobium vibrioforme were studied. The intactness of the isolated reaction centres was assayed from the amount of photoreducible FeS centres and spin-polarized triplet signal as compared to the parent membranes. The results obtained allow us to rationalize conflicting data published for the green sulfur bacterial reaction centres during recent years. The previously reported broad linewidths of the FeS centres in purified reaction centres were shown to be very likely due to detergent induced alterations. For the terminal FeS accepters in purified reaction centres, a (preparation dependent) shift towards less negative redox midpoint potentials with respect to what is seen in the membranes was found. These redox midpoint potentials in intact membrane samples were seen to be significantly more negative than those of the respective FeS centres in Photosystem I from cyanobacteria and chloroplasts. The results demonstrate the high similarity concerning the characteristics of the terminal FeS accepters in different green sulfur bacterial species.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTOSYNTHETIC REACTION-CENTER; REACTION-CENTER COMPLEX; PHOTOSYSTEM-I; CHLOROBIUM-VIBRIOFORME; FES-CENTERS; SUBUNIT; POLYPEPTIDE; LIMICOLA; SPINACH; PROTEIN; Chlorobium; iron-sulfur centres; reaction centre complex |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Günter Hauska |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2023 06:39 |
| Last Modified: | 17 May 2023 06:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51083 |
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