Bachmeier, Manfred and Löffler, Georg (1997) The effect of platelet-derived growth factor and adipogenic hormones on the expression of CCAAT/enhancer-binding proteins in 3T3-L1 cells in serum-free conditions. EUROPEAN JOURNAL OF BIOCHEMISTRY, 243 (1-2). pp. 128-133. ISSN 0014-2956
Full text not available from this repository.Abstract
In the absence of serum and serum substitutes, insulin at high doses together with platelet-derived growth factor BB (PDGF BB), corticosterone, and 3-isobutyl-1-methyl-xanthine is required to stimulate differentiation of 3T3-L1 preadipocytes. Under these conditions the differentiating cells express the CCAAT/enhancer-binding proteins (C/EBP) C/EBP delta, C/EBP delta, and C/EBP alpha with a similar sequence as described for differentiating cells in the presence of serum. The major differences detected tinder serum-free conditions are as follows: (a) PDGF BB is the major stimulator of the expression of the C/EBP beta isoform liver activator protein (LAP). (b) The expression of LAP is also increased in preconfluent, proliferating cells due to the treatment with PDGF BE. (c) A small protein of 20 kDa (p20(C/EBP beta)) is detected with the anti-C/EBP beta antibody. It is synthesized at high levels in such cells, which subsequently express high levels of the differentiation markers C/EBP alpha and glycerol 3-phosphate dehydrogenase. (d) Treatment of cells with fibroblast growth factor-2 (bFGF) in addition to adipogenic hormones results in differentiation and C/EBP alpha expression only to a very moderate extent as compared to treatment with PDGF BE but leads to a strong expression of both C/EBP beta and C/EBP delta.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DIFFERENTIATION-INDUCED GENE; RESPONSIVE GLUCOSE TRANSPORTER; REGULATED NUCLEAR-PROTEIN; C/EBP-ALPHA-GENE; ADIPOCYTE DIFFERENTIATION; ADIPOSE CONVERSION; MESSENGER-RNA; GLYCEROPHOSPHATE DEHYDROGENASE; LEUCINE-ZIPPER; INSULIN; 3T3-L1 preadipocytes; platelet-derived growth factor; adipogenic hormone; CCAAT/enhancer-binding protein; differentiation |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2023 07:58 |
| Last Modified: | 17 May 2023 07:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51098 |
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