Woriedh, Mayada and Merkl, Rainer and Dresselhaus, Thomas (2015) Maize EMBRYO SAC family peptides interact differentially with pollen tubes and fungal cells. JOURNAL OF EXPERIMENTAL BOTANY, 66 (17). pp. 5205-5216. ISSN 0022-0957, 1460-2431
Full text not available from this repository. (Request a copy)Abstract
EMBRYO SAC1-4 (ES1-4) peptides belong to the defensin subgroup of cysteine-rich peptides known to mediate pollen tube burst in Zea mays (maize). ES1-4 are reported here to also be capable of inhibiting germination and growth of the maize fungal pathogens Fusarium graminearum and Ustilago maydis at higher concentrations. Dividing the peptides into smaller pieces showed that a 15-amino-acid peptide located in a highly variable loop region lacking similarity to other defensins or defensin-like peptides binds to maize pollen tube surfaces, causing swelling prior to burst. This peptide fragment and a second conserved neighbouring fragment showed suppression of fungal germination and growth. The two peptides caused swelling of fungal cells, production of reactive oxygen species, and finally the formation of big vacuoles prior to burst at high peptide concentration. Furthermore, peptide fragments were found to bind differently to fungal cells. In necrotrophic F. graminearum, a peptide fragment named ES-d bound only at cell surfaces whereas the peptide ES-c bound at cell surfaces and also accumulated inside cells. Conversely, in biotrophic U. maydis, both peptide fragments accumulated inside cells, but, if applied at higher concentration, ES-c but not ES-d accumulated mainly in vacuoles. Mapping of peptide interaction sites identified amino acids differing in pollen tube burst and fungal response reactions. In summary, these findings indicate that residues targeting pollen tube burst in maize are specific to the ES family, while residues targeting fungal growth are conserved within defensins and defensin-like peptides.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PLANT DEFENSINS; CANDIDA-ALBICANS; USTILAGO-MAYDIS; PORE REGION; ANTIFUNGAL; GENETICS; CHANNELS; IDENTIFICATION; GAMETOPHYTE; INHIBITION; Cysteine rich protein (CRP); defensin; Fusarium graminearum; maize; pollen tube; ROS; Ustilago maydis |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jun 2019 13:24 |
| Last Modified: | 25 Jun 2019 13:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5112 |
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