Sittinger, M and Schultz, O and Keyszer, G and Minuth, WW and Burmester, GR (1997) Artificial tissues in perfusion culture. INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 20 (1). pp. 57-62. ISSN 0391-3988,
Full text not available from this repository.Abstract
In the stagnant environment of traditional culture dishes it is difficult to generate long term experiments or artificial tissues from human cells. For this reason a perfusion culture system with a stable supply of nutrients was developed. Human chondrocytes were seeded three-dimensionally in resorbable polymer fleeces. The cell-polymer tissues were then mounted in newly developed containers (W. W. Minuth et al, Biotechniques, 1996) and continuously perfused by fresh medium for 40 days. Samples from the effluate were analyzed daily and the pH of the medium and glucose concentration remained stable during this period. The lactid acid concentration increased from 0, 17 mg/ml to 0,35 mg/ml, which was influenced by the degradation of the resorbable polymer fibers used as three dimensional support material for the cells. This perfusion system proved to be reliable especially in long term cultures. Any components in the culture medium of the cells could be monitored without disturbances as caused by manual medium replacement. These results suggest the described perfusion culture system to be a valuable and convenient fool for many applications in tissue engineering, especially in the generation of artificial connective tissue.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELLS; CARTILAGE; DIFFERENTIATION; BONE; tissue engineering; perfusion culture; extracellular matrix; chondrocytes; metabolite |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51200 |
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