Kokanova-Nedialkova, Zlatina and Kondeva-Burdina, Magdalena and Zheleva-Dimitrova, Dimitrina and Tzankova, Virginia and Nikolov, Stefan and Heilmann, Joerg and Nedialkov, Paraskev T. (2015) 6-Methoxyflavonol Glycosides with In Vitro Hepatoprotective Activity from Chenopodium bonus-henricus Roots. NATURAL PRODUCT COMMUNICATIONS, 10 (8). pp. 1377-1380. ISSN 1934-578X, 1555-9475
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One new, namely 6-methoxykaempferol 3-O-[beta-apiofuranosyl(1 -> 2)]-beta-glucopyranosyl(1 -> 6)-beta-glucopyranoside (2), and two known flavonoid glycosides, spinacetin 3-O-[beta-apiofuranosyl(1 -> 2)]-beta-glucopyranosyl(1 -> 6)-beta-glucopyranoside (1) and spinacetin 3-O-gentiobioside (3), were isolated from the roots of Chenopodium bonus-henricus L. Their structures were determined by means of spectroscopic methods (1D, 2D NMR, UV, IR) and HR-ESI-MS. Radical scavenging and anti-oxidant activities of 1 and 3 were established using DPPH and ABTS free radicals, FRAP assay and inhibition of lipid peroxidation (LP) in a linoleic acid system by the ferric thiocyanate method. Compound 3 was found to possess stronger DPPH and ABTS radical scavenging activity (IC50 0.44 +/- 0.008 mM and 0.089 +/- 0.002 mM, respectively) compared with 1 (IC50 1.22 +/- 0.010 mM and 0.11 +/- 0.004 mM, respectively). Both flavonoids inhibited the lipid peroxidation of linoleic acid significantly. Additionally, 1 and 3 significantly reduced the cellular damage caused by the hepatotoxic agent CCl4 in rat hepatocytes and preserved cell viability and GSH level, decreased LDH leakage and reduced lipid damage. Effects were similar to those of the positive control silymarin. Control of self-toxic effects made in a MTT based assay using HepG2 cells revealed statistically significant cytotoxic effects only in very high concentrations (exceeding mM) and an incubation time of 72 h, making flavonoid glycosides with a 6-methoxykaempferol skeleton a promising and safe class of hepatoprotective compounds.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLAVONOL GLYCOSIDES; SPINACIA-OLERACEA; ISOLATED HEPATOCYTES; HEPATOTOXICITY; TETRACHLORIDE; MECHANISM; LEAVES; ASSAY; Chenopodium bonus-henricus; Amaranthaceae; 6-Methoxyflavonol glycosides; Anti-oxidant activity; Anti-hepatotoxic activity |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jun 2019 13:52 |
| Last Modified: | 25 Jun 2019 13:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5123 |
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