Pscherer, A and Dorflinger, U and Kirfel, J and Gawlas, K and Ruschoff, J and Buettner, R and Schule, R (1996) The helix-loop-helix transcription factor SEF-2 regulates the activity of a novel Initiator element in the promoter of the human somatostatin receptor II gene. EMBO JOURNAL, 15 (23). pp. 6680-6690. ISSN 0261-4189,
Full text not available from this repository.Abstract
The effects of somatostatin hormones are mediated by a family of five different seven-helix transmembrane spanning receptors (SSTR1-5), The expression of the five different SSTR subtypes displays a complex temporal- and tissue-specific pattern, To investigate the molecular mechanisms controlling the different expression patterns of the SSTRs, we cloned the 5'-flanking region of the human SSTR2 gene, Characterization of the SSTR2 promoter resulted in the identification of a novel initiator element (SSTR2inr). Transcriptional activity of the SSTR2inr is dependent on the presence of a binding site (E-box) for basic helix-loop-helix (bHLH) transcription factors, By screening a mouse brain cDNA expression library we isolated a cDNA coding for the bHLH transcription factor SEF-2, SEF-2 binds to the E-box present in the SSTR2inr, both in vitro and in vivo and activates transcription from the SSTR2inr. A single point mutation within the E-box eliminates binding of SEF-2 and results in a complete loss of transcriptional activity of the SSTR2inr, Furthermore, DNA binding studies demonstrate that the basal transcription factor TFIIB can be tethered to the SSTR2inr through physical interaction with SEF-2, In summary, the SSTR2inr represents a novel type of initiator element that confers gene expression in the absence of a TATA-box or binding sites for other known initiator factors, like YY-1 or USF.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-II; MOLECULAR-CLONING; RESPONSE ELEMENTS; SEQUENCE-ANALYSIS; MAMMALIAN-CELLS; BINDING PROTEIN; TFII-I; RAT; EXPRESSION; PITUITARY; bHLH transcription factors; initiator sequences; protein-protein interaction; somatostatin receptor; transcriptional regulation |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51285 |
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