Ocana, Cristina and Lukic, Sonja and del Valle, Manel (2015) Aptamer-antibody sandwich assay for cytochrome c employing an MWCNT platform and electrochemical impedance. MICROCHIMICA ACTA, 182 (11-12). pp. 2045-2053. ISSN 0026-3672, 1436-5073
Full text not available from this repository. (Request a copy)Abstract
We report on a sensitive aptamer-antibody interaction-based assay for cytochrome c (Cyt c) using electrochemical impedance. 4-Amino benzoic acid is used for the oriented immobilization of aminated aptamers onto multi-walled carbon nanotubes on the surface of a screen-printed electrode via electrochemical grafting. Impedance was measured in a solution containing the redox system ferro/ferricyanide. The change in interfacial charge transfer resistance (R-ct) experienced by the redox marker was recorded to confirm the formation of a complex between aptamer and the target (Cyt c). A biotinylated antibody against cytochrome c was then used in a sandwich type of assay. The addition of streptavidin conjugated to gold nanoparticles and signal enhancement by treatment with silver led to a further increase in Rct. Under optimized conditions, a detection limit as low as 12 pM was obtained. Cross-reactivity against other serum proteins including fibrinogen, BSA and immunoglobulin G demonstrated improved selectivity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHEMICALLY-MODIFIED GRAPHENES; IMPEDIMETRIC APTASENSOR; DNA HYBRIDIZATION; AMPLIFICATION; LABEL; ELECTRODE; PROTEIN; NANOPARTICLES; SPECTROSCOPY; RECOGNITION; Aptamer; Sandwich; Cytochrome c; SEM; Gold-nanoparticles electrochemical impedance spectroscopy |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Jul 2019 11:21 |
| Last Modified: | 03 Jul 2019 11:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5135 |
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