Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI)

Beyser, Kurt and Fabry, Stefan (1996) Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI). FEBS LETTERS, 396 (2-3). pp. 298-304. ISSN 0014-5793, 1873-3468

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Abstract

The cDNA encoding a Ypt/Rab guanosine dissociation inhibitor (Ypt-GDI) was isolated from the multicellular green alga Volvox carteri, representing the first complete plant gdi gene described. The gdiV1 gene occurs as a single copy in the algal genome, indicating that its product regulates all YptV proteins from Volvox. The derived GDI protein (GDIV1p) shows high similarity to animal and fungal GDIs. A specific antibody developed against GDIV1p detected the protein throughout the whole Volvox life-cycle. GDIV1p was localized in the cytoplasm and in the algal flagellum. This is in line with earlier findings of a dual localization of Ypt proteins both in the cell body and in the motility organelle, and indicates a novel role of the GDI/Ypt system, possibly in intraflagellar transport.

Item Type: Article
Uncontrolled Keywords: GTP-BINDING PROTEINS; ALGA VOLVOX-CARTERI; FLAGELLAR MEMBRANE; RAB PROTEINS; YPT GENES; VESICLES; FAMILY; EXPRESSION; TRANSPORT; BIOGENESIS; Ypt/Rab protein; green alga; transport; Volvox; Chlamydomonas; flagellum
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie
Depositing User: Dr. Gernot Deinzer
Date Deposited: 09 Jun 2023 12:52
Last Modified: 09 Jun 2023 12:52
URI: https://pred.uni-regensburg.de/id/eprint/51352

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