Rehli, Michael and Luger, Karin and Beier, Wilfried and Falk, Werner (1996) Molecular cloning and expression of mouse procalcitonin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 226 (2). pp. 420-425. ISSN 0006-291X, 1090-2104
Full text not available from this repository.Abstract
The nucleotide sequence for mouse calcitonin was determined from a cDNA obtained using a polymerase chain reaction (PCR) based method, the rapid amplification of cDNA ends (RACE)-PCR. Primers designed from highly conserved regions in the coding sequences of known rat and human calcitonin cDNAs were used to amplify calcitonin cDNA as 5'-end and 3'-end fragments from mouse thyroid RNA. The obtained cDNA is 850 bp in length most probably representing the entire mouse calcitonin mRNA, It contains an open reading frame coding for a 136 amino acid protein with a calculated M(r) of 15,143. Comparison of the deduced amino acid sequences of preprocalcitonin of mice with other species revealed highest homologies to the rat (93%) and human (77%) sequences. A recombinant form of mouse procalcitonin (rmPCT) of approximately 17 kDa was expressed as a fusion peptide in E.coli transformed with a PCR-cloned expression construct. (C) 1996 Academic Press, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CALCITONIN MESSENGER-RNA; SEQUENCE; GENE |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2023 04:57 |
| Last Modified: | 29 Jun 2023 04:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51477 |
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