Jensen, Boye L. and Schmid, Charlotte and Kurtz, Armin (1996) Prostaglandins stimulate renin secretion and renin mRNA in mouse renal juxtaglomerular cells. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 271 (3). F659-F669. ISSN 0363-6127
Full text not available from this repository.Abstract
This study examined 1) effects of prostaglandins (PG) on renin secretion and renin gene expression from isolated juxtaglomerular granular cells and 2) expression of cyclooxygenases in juxtaglomerular structures. Incubation of granular cell cultures with PGE(2), -I-2, -F2(alpha), and thromboxane B-2 identified PGI(2) and PGE(2) as stimulators of renin secretion; the effects were dose and time dependent. PGE(2) also increased renin mRNA accumulation time and dose dependent. PGE(2) and PGI(2) activated adenylate cyclase concentration dependent in granular cells. PGE(2) stimulations of renin secretion and renin mRNA were nonadditive to those of forskolin and were inhibited by endothelin. The findings are compatible with cellular actions through adenosine 3',5'-cyclic monophosphate (cAMP). On total RNA harvested from whole kidneys, from microdissected glomeruli with attached afferent arterioles and from mesangial cells in primary culture, reverse transcription-polymerase chain reaction revealed significant expression of cyclooxygenase I and II. By direct interaction with PG receptors on renal juxtaglomerular cells, PGE(2) and PGI(2) can act as potent and rapid stimulators of renin secretion and renin mRNA probably through cAMP-dependent pathways.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ISOLATED RAT GLOMERULI; MACULA DENSA; CORTICAL SLICES; GENE-EXPRESSION; E RECEPTOR; RELEASE; KIDNEY; CYCLOOXYGENASE; LOCALIZATION; ARTERIOLES; prostaglandin E(2); prostaglandin F-2; thrombaxone B-2; kidney; cyclooxygenase; adenosine 3',5'-cyclic monophosphate |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jun 2023 11:05 |
| Last Modified: | 22 Jun 2023 11:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51484 |
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