Wrobel, Karl-Heinz and Bickel, Daniela and Schimmel, Margit and Kujat, Richard (1996) Immunohistochemical demonstration of nerve growth factor receptor in bovine testis. CELL AND TISSUE RESEARCH, 285 (2). pp. 189-197. ISSN 0302-766X, 1432-0878
Full text not available from this repository.Abstract
Nerve growth factor receptor (low-affinity form) was demonstrated immunohistochemically in bovine testis by using a monoclonal mouse anti-human antibody. In the 7-month-old fetus and in the early postnatal testis, the peritubular and intertubular fibroblast-like mesenchymal cells showed a strong reaction. Following differentiation of these cells into Leydig and myoid peritubular cells, the nerve growth factor receptor was no longer expressed. However, peritubular and intertubular testicular fibroblasts/fibrocytes, which are also derived from mesenchymal precursors, remained positive. Additionally, the nerve growth factor receptor was demonstrated in postnatal prespermatogonia, A-spermatogonia, I-spermatogonia and members of the spermatogonia precursor cell line; B-spermatogonia remained negative. In A-spermatogonia and I-spermatogonia, the expression of the nerve growth factor receptor was cell-cycle-dependent and was mostly observed during G(1)-phase. Pre-embedding ultrahistochemistry with gold-conjugated antibody followed by silver-enhancement revealed that the nerve growth factor receptor was localized at the outer cell surface. The metal granules showed a regular distribution in positive spermatogonia. In testicular fibroblasts/fibrocytes the long narrow processes were preferentially decorated.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROLIFERATION-RELATED ANTIGEN; HUMAN NGF RECEPTOR; NEUROTROPHIC FACTOR; PROSTATIC ADENOCARCINOMA; POSTNATAL-DEVELOPMENT; MOLECULAR-CLONING; TRK PROTOONCOGENE; KI-67 DETECTS; RAT TESTIS; EXPRESSION; testis; nerve growth factor receptor; immunohistochemistry; ultrastructure; bovine |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2023 08:32 |
| Last Modified: | 29 Jun 2023 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51553 |
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