Göpfert, Thea and Gess, Bernhard and Eckardt, Kai-Uwe and Kurtz, Armin (1996) Hypoxia signalling in the control of erythropoietin gene expression in rat hepatocytes. JOURNAL OF CELLULAR PHYSIOLOGY, 168 (2). pp. 354-361. ISSN 0021-9541
Full text not available from this repository.Abstract
This study aimed to investigate the sequence of events involved in the stimulation of erythropoietin (EPO) gene expression by hypoxia in hepatocytes. To this end, primary cultures of rat hepatocytes were kept at either high (40% O-2) or low (3% O-2) oxygen tensions for 2.5 h. Hypoxia increased EPO mRNA about fifteen-fold, whilst the divalent cation cobalt (50-100 mu M) or the iron chelator desferrioxamine (10-200 mu M) did not increase EPO mRNA levels. Addition of hydrogen peroxide (100-500 mu M) to the culture medium did also not change EPO mRNA levels at high or low oxygen tension. Addition of catalase (50-200 mu g/ml) to the culture medium resulted in a lower level of hypoxia-induced EPO mRNA. Inhibition of protein synthesis by cycloheximide (100 mu M) completely abolished the increase of EPO mRNA in response to hypoxia. Hypoxia but not cobalt increased the appearance of the hypoxia-inducible factor 1 (HIF-1), and this increase was blunted by cycloheximide. Taken together, these findings suggest that a classic heme protein and a related oxygen-dependent production of oxygen radicals is less likely to be involved in the regulation of the EPO gene by oxygen in hepatocytes. On the other hand, intact protein synthesis is an absolute requirement for the hypoxia-induced appearance of HIF-1 and for hypoxia-stimulated expression of the EPO gene in hepatocytes. (C) 1996 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDUCIBLE FACTOR-I; DEPENDENT EXPRESSION; MESSENGER-RNA; OXYGEN SENSOR; ENHANCER; PROTEIN; CELLS; LIVER |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2023 12:24 |
| Last Modified: | 26 Oct 2023 12:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51571 |
Actions (login required)
 |
View Item |
